OPN1MW2 sirna

OPN1MW2 siRNA (Human)

Cat. Num. AAA8202022

• Gene Names: OPN1MW; CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OPN1MW2; OPN1MW2 siRNA (Human); GCP; Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin; GOP; OPN1MW2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: OPN1MW2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 364

• Applicable Applications for OPN1MW2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OPN1MW2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OPN1MW2 sirna

siRNA to inhibit OPN1MW2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503965
• NCBI GeneID: 2652
• NCBI Accession #: NP_000504.1
• NCBI GenBank Nucleotide #: NM_000513.2
• UniProt Accession #: P04001
• Molecular Weight: 40,584 Da
• NCBI Official Full Name: medium-wave-sensitive opsin 1
• NCBI Official Synonym Full Names: opsin 1 (cone pigments), medium-wave-sensitive
• NCBI Official Symbol: OPN1MW
• NCBI Official Synonym Symbols: CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• NCBI Protein Information: medium-wave-sensitive opsin 1
• UniProt Protein Name: Medium-wave-sensitive opsin 1
• Protein Family: Medium-wave-sensitive opsin
• UniProt Gene Name: OPN1MW
• UniProt Synonym Gene Names: GCP; GOP
• UniProt Entry Name: OPSG_HUMAN

NCBI Description

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness. [provided by RefSeq, Mar 2009]

Uniprot Description

OPN1MW: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Defects in OPN1MW are the cause of partial colorblindness deutan series (CBD); also known as deuteranopia. Defects in OPN1MW are a cause of blue cone monochromacy (BCM). A rare X-linked congenital stationary cone dysfunction syndrome characterized by the absence of functional long wavelength-sensitive and medium wavelength-sensitive cones in the retina. Color discrimination is severely impaired from birth, and vision is derived from the remaining preserved blue (S) cones and rod photoreceptors. BCM typically presents with reduced visual acuity, pendular nystagmus, and photophobia. Patients often have myopia. Defects in OPN1MW are a cause of cone dystrophy type 5 (COD5). A X-linked cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; photoreceptor activity

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; phototransduction, visible light; visual perception; retinoid metabolic process; protein-chromophore linkage

Disease: Blue Cone Monochromacy; Colorblindness, Partial, Deutan Series