OBSL1 sirna

OBSL1 siRNA (Human)

Cat. Num. AAA8213713

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OBSL1; OBSL1 siRNA (Human); KIAA0657; Obscurin-like protein 1; OBSL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: OBSL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1025

• Applicable Applications for OBSL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OBSL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OBSL1 sirna

siRNA to inhibit OBSL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 291084513
• NCBI GeneID: 23363
• NCBI Accession #: NP_001166879.1
• NCBI GenBank Nucleotide #: NM_001173408.1
• UniProt Accession #: O75147; Q96IW3; A4KVA4; A4KVA5; S4R3M6
• Molecular Weight: 168,521 Da
• NCBI Official Full Name: obscurin-like protein 1 isoform 3
• NCBI Official Synonym Full Names: obscurin-like 1
• NCBI Official Symbol: OBSL1
• NCBI Protein Information: obscurin-like protein 1
• UniProt Protein Name: Obscurin-like protein 1
• Protein Family: Obscurin-like protein
• UniProt Gene Name: OBSL1
• UniProt Synonym Gene Names: KIAA0657
• UniProt Entry Name: OBSL1_HUMAN

NCBI Description

Cytoskeletal adaptor proteins function in linking the internal cytoskeleton of cells to the cell membrane. This gene encodes a cytoskeletal adaptor protein, which is a member of the Unc-89/obscurin family. The protein contains multiple N- and C-terminal immunoglobulin (Ig)-like domains and a central fibronectin type 3 domain. Mutations in this gene cause 3M syndrome type 2. Alternatively spliced transcript variants encoding different isoforms have been found in this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

OBSL1: Defects in OBSL1 are the cause of 3M syndrome type 2 (3M2). An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: Golgi apparatus; centrosome; perinuclear region of cytoplasm; cytoplasm; M band; Z disc

Molecular Function: protein binding; cytoskeletal adaptor activity

Biological Process: protein localization in Golgi apparatus; regulation of mitosis; cardiac myofibril assembly; cytoskeleton organization and biogenesis; positive regulation of dendrite morphogenesis; microtubule cytoskeleton organization and biogenesis; Golgi organization and biogenesis

Disease: Three M Syndrome 2