NUTF2 sirna

NUTF2 siRNA (Human)

Cat. Num. AAA8215398

• Gene Names: NUTF2; NTF2; PP15
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUTF2; NUTF2 siRNA (Human); NTF2; Nuclear transport factor 2; NTF-2; Placental protein 15; PP15; NUTF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUTF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 127

• Applicable Applications for NUTF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUTF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUTF2 sirna

siRNA to inhibit NUTF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5031985
• NCBI GeneID: 10204
• NCBI Accession #: NP_005787.1
• NCBI GenBank Nucleotide #: NM_005796.1
• UniProt Accession #: P61970; P13662; Q6IB67; B2R4G7
• Molecular Weight: 14,478 Da
• NCBI Official Full Name: nuclear transport factor 2
• NCBI Official Synonym Full Names: nuclear transport factor 2
• NCBI Official Symbol: NUTF2
• NCBI Official Synonym Symbols: NTF2; PP15
• NCBI Protein Information: nuclear transport factor 2
• UniProt Protein Name: Nuclear transport factor 2
• UniProt Gene Name: NUTF2
• UniProt Synonym Gene Names: NTF2; NTF-2; PP15
• UniProt Entry Name: NTF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. [provided by RefSeq, Jul 2008]

Uniprot Description

NUTF2: Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. Homodimer.

Protein type: Nuclear import

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nuclear pore; cytosol

Molecular Function: protein binding; transporter activity

Biological Process: protein export from nucleus