Rabbit ABL2 Polyclonal Antibody | anti-ABL2 antibody

ABL2 Polyclonal Antibody

Cat. Num. AAA3012184

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: ABL2; Polyclonal Antibody; ABL2 Polyclonal Antibody; Abelson murine leukemia viral oncogene homolog 2; Abelson related gene protein; Abelson tyrosine-protein kinase 2; Abelson-related gene protein; ABLL; ARG; Tyrosine kinase ARG; Tyrosine protein kinase ABL2; Tyrosine-protein kinase ARG; v abl Abelson murine leukemia viral oncogene homolog 2; anti-ABL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABL2 polyclonal antibody detects endogenous levels of ABL2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1043

• Applicable Applications for anti-ABL2 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:2,000; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of ABL2 on different lysates using anti-ABL2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: Jurkat)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Raji cells with ABL2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Related Product Information for anti-ABL2 antibody

The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including chronic myelocytic leukemia (CML), in which it undergoes a (9;22) chromosomal translocation and produces the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein tyrosine kinase. The related protein tyrosine kinase Arg, also designated Abl2, contains an SH2 and an SH3 domain. Arg has been shown to interact with and to phosphorylate c-Crk.

NCBI and Uniprot Product Information

• NCBI GI #: 269847793
• NCBI GeneID: 27
• NCBI Accession #: NP_001161711.1
• NCBI GenBank Nucleotide #: NP_001161711.1
• UniProt Accession #: P42684; Q4JIM5
• Molecular Weight: 128kDa
• NCBI Official Full Name: tyrosine-protein kinase ABL2 isoform i
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: tyrosine-protein kinase ABL2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Oncoprotein; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; actin cytoskeleton; phagocytic cup

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; positive regulation of protein binding; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of apoptosis; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; regulation of endocytosis; visual learning; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; actin filament bundle formation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; protein modification process; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; negative regulation of Rho protein signal transduction; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuron remodeling