NUDT7 sirna

NUDT7 siRNA (Human)

Cat. Num. AAA8235862

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUDT7; NUDT7 siRNA (Human); Peroxisomal coenzyme A diphosphatase NUDT7; Nucleoside diphosphate-linked moiety X motif 7; Nudix motif 7; NUDT7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUDT7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 238

• Applicable Applications for NUDT7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUDT7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUDT7 sirna

siRNA to inhibit NUDT7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157785656
• NCBI GeneID: 283927
• NCBI Accession #: NP_001099133.1
• NCBI GenBank Nucleotide #: NM_001105663.2
• UniProt Accession #: P0C024; B4DLE5; H3BUB8
• Molecular Weight: 18,724 Da
• NCBI Official Full Name: peroxisomal coenzyme A diphosphatase NUDT7 isoform 1
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 7
• NCBI Official Symbol: NUDT7
• NCBI Protein Information: peroxisomal coenzyme A diphosphatase NUDT7
• UniProt Protein Name: Peroxisomal coenzyme A diphosphatase NUDT7
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT7
• UniProt Synonym Gene Names: Nudix motif 7
• UniProt Entry Name: NUDT7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Nudix hydrolase family. Nudix hydrolases eliminate potentially toxic nucleotide metabolites from the cell and regulate the concentrations and availability of many different nucleotide substrates, cofactors, and signaling molecules. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

NUDT7: Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. CoA into 3',5'-ADP and 4'- phosphopantetheine. Has no activity toward NDP-sugars, CDP- alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Belongs to the Nudix hydrolase family. PCD1 subfamily.

Protein type: EC 3.6.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: peroxisome

Molecular Function: snoRNA binding; m7G(5')pppN diphosphatase activity; manganese ion binding; magnesium ion binding; acetyl-CoA hydrolase activity; receptor binding

Biological Process: acetyl-CoA catabolic process; brown fat cell differentiation; nucleoside diphosphate metabolic process; coenzyme A catabolic process