NSMCE2 sirna

NSMCE2 siRNA (Human)

Cat. Num. AAA8241116

• Gene Names: NSMCE2; NSE2; MMS21; ZMIZ7; C8orf36
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSMCE2; NSMCE2 siRNA (Human); C8orf36; MMS21; E3 SUMO-protein ligase NSE2; MMS21 homolog; hMMS21; Non-structural maintenance of chromosomes element 2 homolog; Non-SMC element 2 homolog; NSMCE2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NSMCE2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 247

• Applicable Applications for NSMCE2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSMCE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSMCE2 sirna

siRNA to inhibit NSMCE2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27734761
• NCBI GeneID: 286053
• NCBI Accession #: NP_775956.1
• NCBI GenBank Nucleotide #: NM_173685.2
• UniProt Accession #: Q96MF7; Q8N549
• Molecular Weight: 27,932 Da
• NCBI Official Full Name: E3 SUMO-protein ligase NSE2
• NCBI Official Synonym Full Names: NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase
• NCBI Official Symbol: NSMCE2
• NCBI Official Synonym Symbols: NSE2; MMS21; ZMIZ7; C8orf36
• NCBI Protein Information: E3 SUMO-protein ligase NSE2
• UniProt Protein Name: E3 SUMO-protein ligase NSE2
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: NSMCE2
• UniProt Synonym Gene Names: C8orf36; MMS21; hMMS21; Non-SMC element 2 homolog
• UniProt Entry Name: NSE2_HUMAN

Uniprot Description

NSMCE2: E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. Belongs to the NSE2 family.

Protein type: EC 6.3.2.-; SUMO conjugating system; EC 6.-.-.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: chromosome, telomeric region; PML body; nucleus

Molecular Function: protein binding; zinc ion binding; SUMO ligase activity; ligase activity

Biological Process: mitosis; protein sumoylation; positive regulation of maintenance of mitotic sister chromatid cohesion; cell division; telomere maintenance via recombination; positive regulation of mitotic metaphase/anaphase transition; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining