NME2 sirna

NME2 siRNA (Human)

Cat. Num. AAA8218246

• Gene Names: NME1-NME2; NMELV; NM23-LV
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NME2; NME2 siRNA (Human); NM23B; Nucleoside diphosphate kinase B; NDK B; NDP kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2; NME2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NME2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 267

• Applicable Applications for NME2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NME2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NME2 sirna

siRNA to inhibit NME2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 66392203
• NCBI GeneID: 654364
• NCBI Accession #: NP_001018146.1
• NCBI GenBank Nucleotide #: NM_001018136.2
• UniProt Accession #: P22392; Q1WM23; Q6LCT6; A8MWA3
• Molecular Weight: 30,137 Da
• NCBI Official Full Name: NME1-NME2 protein
• NCBI Official Synonym Full Names: NME1-NME2 readthrough
• NCBI Official Symbol: NME1-NME2
• NCBI Official Synonym Symbols: NMELV; NM23-LV
• NCBI Protein Information: NME1-NME2 protein
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: NME2
• UniProt Synonym Gene Names: NM23B; NDK B; NDP kinase B
• UniProt Entry Name: NDKB_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; EC 2.7.13.3; Nuclear receptor co-regulator; EC 2.7.4.6; Nucleotide Metabolism - purine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; lamellipodium; perinuclear region of cytoplasm; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; gamma-tubulin binding; metal ion binding; protein histidine kinase activity; intermediate filament binding; nucleoside diphosphate kinase activity; protein kinase binding; transcription factor activity; single-stranded DNA binding; ATP binding

Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; negative regulation of myeloid leukocyte differentiation; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; regulation of epidermis development; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; negative regulation of apoptosis; response to amine stimulus; positive regulation of keratinocyte differentiation