NHEJ1 sirna

NHEJ1 siRNA (Human)

Cat. Num. AAA8238361

• Gene Names: NHEJ1; XLF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NHEJ1; NHEJ1 siRNA (Human); XLF; Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor; NHEJ1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NHEJ1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 299

• Applicable Applications for NHEJ1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NHEJ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NHEJ1 sirna

siRNA to inhibit NHEJ1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13376142
• NCBI GeneID: 79840
• NCBI Accession #: NP_079058.1
• NCBI GenBank Nucleotide #: NM_024782.2
• UniProt Accession #: Q9H9Q4; Q4ZFW7; Q6IA64; Q96JS9; B8ZZA4
• Molecular Weight: 35,291 Da
• NCBI Official Full Name: non-homologous end-joining factor 1
• NCBI Official Synonym Full Names: nonhomologous end-joining factor 1
• NCBI Official Symbol: NHEJ1
• NCBI Official Synonym Symbols: XLF
• NCBI Protein Information: non-homologous end-joining factor 1
• UniProt Protein Name: Non-homologous end-joining factor 1
• Protein Family: Non-homologous end-joining factor
• UniProt Gene Name: NHEJ1
• UniProt Synonym Gene Names: XLF
• UniProt Entry Name: NHEJ1_HUMAN

NCBI Description

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding

Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; DNA recombination; T cell differentiation

Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation