NFX1 sirna

NFX1 siRNA (Human)

Cat. Num. AAA8204075

• Gene Names: NFX1; NFX2; Tex42; TEG-42
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NFX1; NFX1 siRNA (Human); NFX2; Transcriptional repressor NF-X1; Nuclear transcription factor. X box-binding protein 1; NFX1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NFX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1120

• Applicable Applications for NFX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NFX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NFX1 sirna

siRNA to inhibit NFX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21361137
• NCBI GeneID: 4799
• NCBI Accession #: NP_002495.2
• NCBI GenBank Nucleotide #: NM_002504.4
• UniProt Accession #: Q12986; Q5VXW6; Q96EL5; Q9BXI1; A8K6H8
• Molecular Weight: 92,679 Da
• NCBI Official Full Name: transcriptional repressor NF-X1 isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor, X-box binding 1
• NCBI Official Symbol: NFX1
• NCBI Official Synonym Symbols: NFX2; Tex42; TEG-42
• NCBI Protein Information: transcriptional repressor NF-X1
• UniProt Protein Name: Transcriptional repressor NF-X1
• Protein Family: NFX1-type zinc finger-containing protein
• UniProt Gene Name: NFX1
• UniProt Synonym Gene Names: NFX2
• UniProt Entry Name: NFX1_HUMAN

NCBI Description

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

NFX1: Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. Belongs to the NFX1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: zinc ion binding; transcription factor activity; ligase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; inflammatory response