NDUFC2-KCTD14 sirna

NDUFC2-KCTD14 siRNA (Human)

Cat. Num. AAA827486

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDUFC2-KCTD14; NDUFC2-KCTD14 siRNA (Human); NADH dehydrogenase [ubiquinone] 1 subunit C2 isoform 2; NDUFC2-KCTD14 readthrough transcript protein; NDUFC2-KCTD14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDUFC2-KCTD14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 114

• Applicable Applications for NDUFC2-KCTD14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDUFC2-KCTD14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDUFC2-KCTD14 sirna

siRNA to inhibit NDUFC2-KCTD14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 323276586
• NCBI GeneID: 100532726
• NCBI Accession #: NP_001190190.1
• NCBI GenBank Nucleotide #: NM_001203261.1
• UniProt Accession #: E9PQ53
• Molecular Weight: 29,591 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NDUFC2-KCTD14 readthrough
• NCBI Official Symbol: NDUFC2-KCTD14
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 subunit C2, isoform 2
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 subunit C2, isoform 2
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFC2-KCTD14
• UniProt Entry Name: NDUCR_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring NDUFC2 (NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa) and KCTD14 (potassium channel tetramerisation domain containing 14) genes on chromosome 11. The read-through transcripts share sequence identity with the upstream gene product and one variant has a frameshifted C-terminal region derived from the downstream gene exons. [provided by RefSeq, Feb 2011]

Uniprot Description

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone ().