NDN sirna

NDN siRNA (Human)

Cat. Num. AAA8222502

• Gene Names: NDN; PWCR; HsT16328
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDN; NDN siRNA (Human); Necdin; NDN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 321

• Applicable Applications for NDN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDN sirna

siRNA to inhibit NDN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505349
• NCBI GeneID: 4692
• NCBI Accession #: NP_002478.1
• NCBI GenBank Nucleotide #: NM_002487.2
• UniProt Accession #: Q99608; B2R6Z5
• Molecular Weight: 36,086 Da
• NCBI Official Full Name: necdin
• NCBI Official Synonym Full Names: necdin, melanoma antigen (MAGE) family member
• NCBI Official Symbol: NDN
• NCBI Official Synonym Symbols: PWCR; HsT16328
• NCBI Protein Information: necdin
• UniProt Protein Name: Necdin
• Protein Family: Necdin
• UniProt Gene Name: NDN
• UniProt Entry Name: NECD_HUMAN

NCBI Description

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008]

Uniprot Description

NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q11.2-q12

Cellular Component: centrosome; cell projection; perikaryon; nucleus; cytosol

Molecular Function: gamma-tubulin binding; DNA binding

Biological Process: nervous system development; central nervous system development; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; axon extension; axonal fasciculation; neuron migration; multicellular organismal homeostasis; glial cell migration; sensory perception of pain; post-embryonic development; respiratory system process; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; regulation of growth

Disease: Prader-willi Syndrome