NCOA6 sirna

NCOA6 siRNA (Human)

Cat. Num. AAA8204199

• Gene Names: NCOA6; NRC; AIB3; ASC2; PRIP; TRBP; RAP250
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCOA6; NCOA6 siRNA (Human); AIB3; KIAA0181; RAP250; TRBP; Nuclear receptor coactivator 6; Activating signal cointegrator 2; ASC-2; Amplified in breast cancer protein 3; Cancer-amplified transcriptional coactivator ASC-2; Nuclear receptor coactivator RAP250; NRC RAP250; Nuclear receptor-activating protein. 250 kDa; Peroxisome proliferator-activated receptor-interacting protein; PPAR-interacting protein; PRIP; Thyroid hormone receptor-binding protein; NCOA6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NCOA6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1070

• Applicable Applications for NCOA6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCOA6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCOA6 sirna

siRNA to inhibit NCOA6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 335892792
• NCBI GeneID: 23054
• NCBI Accession #: NP_001229468.1
• NCBI GenBank Nucleotide #: NM_001242539.1
• UniProt Accession #: Q14686; Q9NTZ9; Q9UH74; Q9UK86; A6NLF1; B2RMN5; E1P5P7
• Molecular Weight: 219,145 Da
• NCBI Official Full Name: nuclear receptor coactivator 6 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor coactivator 6
• NCBI Official Symbol: NCOA6
• NCBI Official Synonym Symbols: NRC; AIB3; ASC2; PRIP; TRBP; RAP250
• NCBI Protein Information: nuclear receptor coactivator 6
• UniProt Protein Name: Nuclear receptor coactivator 6
• Protein Family: Nuclear receptor coactivator
• UniProt Gene Name: NCOA6
• UniProt Synonym Gene Names: AIB3; KIAA0181; RAP250; TRBP; ASC-2; NRC RAP250; PPAR-interacting protein; PRIP
• UniProt Entry Name: NCOA6_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional coactivator that can interact with nuclear hormone receptors to enhance their transcriptional activator functions. This protein has been shown to be involved in the hormone-dependent coactivation of several receptors, including prostanoid, retinoid, vitamin D3, thyroid hormone, and steroid receptors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

NCOA6: a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Ubiquitous. Highly expressed in brain, prostate, testis and ovary. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of nuclear receptors for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Interacts in vitro with the basal transcription factors TFIIA and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferases p300 and CBP, and with the methyltransferases NCOA6IP and PRMT2. Phosphorylation of S884 inhibits the binding to the ERs.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; transcription factor complex; histone methyltransferase complex; intracellular membrane-bound organelle; nucleus

Molecular Function: retinoid X receptor binding; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; estrogen receptor binding; chromatin binding; thyroid hormone receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; transcription initiation; response to hormone stimulus; cellular lipid metabolic process; DNA repair; DNA recombination; glucocorticoid receptor signaling pathway; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; brain development; DNA replication; response to DNA damage stimulus