MYL12A sirna

MYL12A siRNA (Human)

Cat. Num. AAA8208679

• Gene Names: MYL12A; MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYL12A; MYL12A siRNA (Human); MLCB; MRLC3; RLC; Myosin regulatory light chain 12A; Epididymis secretory protein Li 24; HEL-S-24; MLC-2B; Myosin RLC; Myosin regulatory light chain 2. nonsarcomeric; Myosin regulatory light chain MRLC3; MYL12A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYL12A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 171

• Applicable Applications for MYL12A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYL12A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYL12A sirna

siRNA to inhibit MYL12A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 740087125
• NCBI GeneID: 10627
• NCBI Accession #: NP_001289976.1
• NCBI GenBank Nucleotide #: NM_001303047.1
• UniProt Accession #: P19105; Q53X45
• Molecular Weight: 19,794 Da
• NCBI Official Full Name: myosin regulatory light chain 12A isoform 1
• NCBI Official Synonym Full Names: myosin, light chain 12A, regulatory, non-sarcomeric
• NCBI Official Symbol: MYL12A
• NCBI Official Synonym Symbols: MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24
• NCBI Protein Information: myosin regulatory light chain 12A
• UniProt Protein Name: Myosin regulatory light chain 12A
• Protein Family: Myosin regulatory
• UniProt Gene Name: MYL12A
• UniProt Synonym Gene Names: MLCB; MRLC3; RLC; HEL-S-24
• UniProt Entry Name: ML12A_HUMAN

NCBI Description

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]

Uniprot Description

MRLC3: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: myosin II complex; stress fiber; cytosol; Z disc

Molecular Function: protein binding; glutamate receptor binding; calcium ion binding

Biological Process: axon guidance; regulation of cell shape; ephrin receptor signaling pathway