MYBPC3 sirna

MYBPC3 siRNA (Human)

Cat. Num. AAA8239052

• Gene Names: MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYBPC3; MYBPC3 siRNA (Human); Myosin-binding protein C. cardiac-type; Cardiac MyBP-C; C-protein. cardiac muscle isoform; MYBPC3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYBPC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1274

• Applicable Applications for MYBPC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYBPC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYBPC3 sirna

siRNA to inhibit MYBPC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148596957
• NCBI GeneID: 4607
• NCBI Accession #: NP_000247.2
• NCBI GenBank Nucleotide #: NM_000256.3
• UniProt Accession #: Q14896; Q16410; Q6R2F7; Q9UE27; Q9UM53; A5PL00
• Molecular Weight: 140,703 Da
• NCBI Official Full Name: myosin-binding protein C, cardiac-type
• NCBI Official Synonym Full Names: myosin binding protein C, cardiac
• NCBI Official Symbol: MYBPC3
• NCBI Official Synonym Symbols: FHC; CMH4; CMD1MM; LVNC10; MYBP-C
• NCBI Protein Information: myosin-binding protein C, cardiac-type
• UniProt Protein Name: Myosin-binding protein C, cardiac-type
• Protein Family: Myosin-binding protein
• UniProt Gene Name: MYBPC3
• UniProt Synonym Gene Names: Cardiac MyBP-C
• UniProt Entry Name: MYPC3_HUMAN

NCBI Description

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family.

Protein type: Myosin-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band

Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding

Biological Process: heart morphogenesis; positive regulation of ATPase activity; regulation of heart rate; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; cell adhesion; myosin filament assembly; cardiac muscle contraction; muscle filament sliding

Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4