Rabbit MYBPC3 ELISA Kit | MYBPC3 elisa kit

Rabbit MYBPC3 (Myosin Binding Protein C, Cardiac) ELISA Kit

Cat. Num. AAA2500205

• Gene Names: MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
• Reactivity: Rabbit
• Synonyms: MYBPC3; Rabbit MYBPC3 (Myosin Binding Protein C; Cardiac) ELISA Kit; MYBPC3 elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit MYBPC3. No significant cross-reactivity or interference between Rabbit MYBPC3 and analogues was observed.
• Sequence Length: 1274

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYBPC3 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit MYBPC3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MYBPC3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MYBPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MYBPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MYBPC3. You can calculate the concentration of MYBPC3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148596957
• NCBI GeneID: 4607
• NCBI Accession #: NP_000247.2
• NCBI GenBank Nucleotide #: NM_000256.3
• UniProt Accession #: Q14896; Q16410; Q6R2F7; Q9UE27; Q9UM53; A5PL00
• Molecular Weight: 140,703 Da
• NCBI Official Full Name: myosin-binding protein C, cardiac-type
• NCBI Official Synonym Full Names: myosin binding protein C, cardiac
• NCBI Official Symbol: MYBPC3
• NCBI Official Synonym Symbols: FHC; CMH4; CMD1MM; LVNC10; MYBP-C
• NCBI Protein Information: myosin-binding protein C, cardiac-type; C-protein, cardiac muscle isoform
• UniProt Protein Name: Myosin-binding protein C, cardiac-type
• Protein Family: Myosin-binding protein
• UniProt Gene Name: MYBPC3
• UniProt Synonym Gene Names: Cardiac MyBP-C
• UniProt Entry Name: MYPC3_HUMAN

NCBI Description

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family.

Protein type: Myosin-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band

Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding

Biological Process: heart morphogenesis; positive regulation of ATPase activity; regulation of heart rate; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; myosin filament assembly; cell adhesion; cardiac muscle contraction; muscle filament sliding

Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4