MUC4 sirna

MUC4 siRNA (Human)

Cat. Num. AAA8230089

• Gene Names: MUC4; ASGP; MUC-4; HSA276359
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MUC4; MUC4 siRNA (Human); Mucin-4; MUC-4; Ascites sialoglycoprotein; ASGP; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucin; MUC4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MUC4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1176

• Applicable Applications for MUC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MUC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MUC4 sirna

siRNA to inhibit MUC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 112382233
• NCBI GeneID: 4585
• NCBI Accession #: NP_004523.3
• NCBI GenBank Nucleotide #: NM_004532.5
• UniProt Accession #: Q99102; O95938; Q9GZM2; Q9GZV6; Q9H481; Q9H482; Q9H483; Q9H484; Q9H485; Q9H486; Q9H487; Q9H4D6
• Molecular Weight: 206,605 Da
• NCBI Official Full Name: mucin-4 isoform d
• NCBI Official Synonym Full Names: mucin 4, cell surface associated
• NCBI Official Symbol: MUC4
• NCBI Official Synonym Symbols: ASGP; MUC-4; HSA276359
• NCBI Protein Information: mucin-4
• UniProt Protein Name: Mucin-4
• Protein Family: Mucin
• UniProt Gene Name: MUC4
• UniProt Synonym Gene Names: MUC-4; ASGP; ASGP-1; ASGP-2
• UniProt Entry Name: MUC4_HUMAN

NCBI Description

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

Uniprot Description

MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: proteinaceous extracellular matrix; extracellular space; membrane; integral to plasma membrane; Golgi lumen; vesicle

Molecular Function: ErbB-2 class receptor binding; extracellular matrix constituent, lubricant activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; cell-matrix adhesion; post-translational protein modification