MTPAP sirna

MTPAP siRNA (Human)

Cat. Num. AAA8211585

• Gene Names: MTPAP; PAPD1; SPAX4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTPAP; MTPAP siRNA (Human); PAPD1; Poly(A) RNA polymerase mitochondrial; PAP; PAP-associated domain-containing protein 1; Polynucleotide adenylyltransferase; Terminal uridylyltransferase 1; TUTase 1; mtPAP; MTPAP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MTPAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 582

• Applicable Applications for MTPAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTPAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTPAP sirna

siRNA to inhibit MTPAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190194365
• NCBI GeneID: 55149
• NCBI Accession #: NP_060579.3
• NCBI GenBank Nucleotide #: NM_018109.3
• UniProt Accession #: Q9NVV4; Q659E3; Q6P7E5; Q9HA74; D3DRX0
• Molecular Weight: 78,833 Da
• NCBI Official Full Name: poly(A) RNA polymerase, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial poly(A) polymerase
• NCBI Official Symbol: MTPAP
• NCBI Official Synonym Symbols: PAPD1; SPAX4
• NCBI Protein Information: poly(A) RNA polymerase, mitochondrial
• UniProt Protein Name: Poly(A) RNA polymerase, mitochondrial
• Protein Family: Poly(A) RNA polymerase
• UniProt Gene Name: MTPAP
• UniProt Synonym Gene Names: PAPD1; PAP; TUTase 1
• UniProt Entry Name: PAPD1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.[provided by RefSeq, Jan 2011]

Uniprot Description

PAPD1: Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4). A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105). Belongs to the DNA polymerase type-B-like family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Transferase; EC 2.7.7.19

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: mitochondrion; intracellular membrane-bound organelle

Molecular Function: identical protein binding; polynucleotide adenylyltransferase activity; protein binding; protein homodimerization activity; manganese ion binding; magnesium ion binding; ATP binding

Biological Process: transcription, DNA-dependent; mRNA polyadenylation

Disease: Spastic Ataxia 4, Autosomal Recessive