Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MTPAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit anti-Human, Mouse MTPAP Polyclonal Antibody | anti-MTPAP antibody
MTPAP Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MTPAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MTPAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NCBI and Uniprot Product Information
Uniprot Description
PAPD1: Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4). A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105). Belongs to the DNA polymerase type-B-like family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.7.19; RNA processing; Transferase
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: mitochondrion; intracellular membrane-bound organelle
Molecular Function: polynucleotide adenylyltransferase activity; identical protein binding; protein binding; protein homodimerization activity; manganese ion binding; magnesium ion binding; ATP binding
Biological Process: transcription, DNA-dependent; mRNA polyadenylation
Disease: Spastic Ataxia 4, Autosomal Recessive
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Product Notes
The MTPAP mtpap (Catalog #AAA9142959) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTPAP Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MTPAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MTPAP mtpap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVIFFLQRRS PPILPTLDSL KTLADAEDKC VIEGNNCTFV RDLSRIKPSQ NTETLELLLK EFFEYFGNFA FDKNSINIRQ GREQNKPDSS PLYIQNPFET SLNISKNVSQ SQLQKFVDLA RESAWILQQE DTDRPSISSN RPWGLVSLLL PSAPNRKSFT KKKSNKFAIE TVKNLLESLK GNRTENFTKT SGKRTISTQT. It is sometimes possible for the material contained within the vial of "MTPAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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