MRVI1 sirna

MRVI1 siRNA (Human)

Cat. Num. AAA8212890

• Gene Names: MRVI1; IRAG; JAW1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MRVI1; MRVI1 siRNA (Human); IRAG; JAW1L; Protein MRVI1; Inositol 1.4.5-trisphosphate receptor-associated cGMP kinase substrate; JAW1-related protein MRVI1; MRVI1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MRVI1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 904

• Applicable Applications for MRVI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MRVI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MRVI1 sirna

siRNA to inhibit MRVI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 332634572
• NCBI GeneID: 10335
• NCBI Accession #: NP_001092049.2
• NCBI GenBank Nucleotide #: NM_001098579.2
• UniProt Accession #: Q9Y6F6; Q17S00; Q9UNY1; B7Z3T4; B7Z6I2; B7Z9A3; E9PQY6; F5H6A1; J3KQZ7
• Molecular Weight: 97,957 Da
• NCBI Official Full Name: protein MRVI1 isoform a
• NCBI Official Synonym Full Names: murine retrovirus integration site 1 homolog
• NCBI Official Symbol: MRVI1
• NCBI Official Synonym Symbols: IRAG; JAW1L
• NCBI Protein Information: protein MRVI1
• UniProt Protein Name: Protein MRVI1
• Protein Family: Protein
• UniProt Gene Name: MRVI1
• UniProt Synonym Gene Names: IRAG; JAW1L
• UniProt Entry Name: MRVI1_HUMAN

NCBI Description

This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011]

Uniprot Description

MRVI1: Plays a role as NO/PRKG1-dependent regulator of IP3- induced calcium release; its phosphorylation by PRKG1 inhibits bradykinin and IP3-induced calcium release from intracellular stores. Recruits PRKG1 to the endoplasmic reticulum and may mediate the assembly of PRKG1 and ITPR1 in a macrocomplex. Involved in PRKG1 signaling cascade leading to inhibition of platelet activation and aggregation. Mediates also NO-dependent inhibition of calcium signaling in gastrointestinal smooth muscle contributing to NO-dependent relaxation. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane

Molecular Function: protein binding

Biological Process: relaxation of vascular smooth muscle; cGMP-mediated signaling; blood coagulation