MLLT10 sirna

MLLT10 siRNA (Human)

Cat. Num. AAA8207948

• Gene Names: MLLT10; AF10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MLLT10; MLLT10 siRNA (Human); AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein; MLLT10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MLLT10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 179

• Applicable Applications for MLLT10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MLLT10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MLLT10 sirna

siRNA to inhibit MLLT10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 307548832
• NCBI GeneID: 8028
• NCBI Accession #: NP_001182555.1
• NCBI GenBank Nucleotide #: NM_001195626.1
• UniProt Accession #: P55197; Q5JT37; Q5VX90; Q66K63; B1ANA8
• Molecular Weight: 20,047 Da
• NCBI Official Full Name: protein AF-10 isoform c
• NCBI Official Synonym Full Names: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
• NCBI Official Symbol: MLLT10
• NCBI Official Synonym Symbols: AF10
• NCBI Protein Information: protein AF-10
• UniProt Protein Name: Protein AF-10
• Protein Family: Protein
• UniProt Gene Name: MLLT10
• UniProt Synonym Gene Names: AF10
• UniProt Entry Name: AF10_HUMAN

NCBI Description

This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

Disease: Leukemia, Acute Myeloid