Rabbit anti-Human PTDSS1 Polyclonal Antibody | anti-PTDSS1 antibody

PTDSS1, CT (PTDSS1, KIAA0024, PSSA, Phosphatidylserine synthase 1, Serine-exchange enzyme I) (Biotin)

Cat. Num. AAA6338471

• Gene Names: PTDSS1; LMHD; PSS1; PSSA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTDSS1; Polyclonal Antibody; CT (PTDSS1; KIAA0024; PSSA; Phosphatidylserine synthase 1; Serine-exchange enzyme I) (Biotin); Serine-exchange enzyme I; anti-PTDSS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PTDSS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTDSS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the C-terminal region of human PTDSS1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTDSS1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PTDSS1 antibody detected the PTDSS1 protein (arrow).)

Related Product Information for anti-PTDSS1 antibody

Phosphatidylserine (PS) accounts for 5 to 10% of cell membrane phospholipids. In addition to its role as a structural component, PS is involved in cell signaling, blood coagulation, and apoptosis. PS is synthesized by a calcium-dependent base-exchange reaction catalyzed by PS synthases (EC 2.7.8.8), like PTDSS1, that exchange L-serine for the polar head group of phosphatidylcholine (PC) or phosphatidylethanolamine (PE) (Sturbois-Balcerzak et al., 2001 [PubMed 11084049]).

Product Categories/Family for anti-PTDSS1 antibody

Antibodies; KIAA; orf

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Tomohiro, S., et al. Biochem. J. 418(2):421-429(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Sturbois-Balcerzak, B., et al. J. Biol. Chem. 276(11):8205-8212(2001). Stone, S.J., et al. J. Biol. Chem. 275(44):34534-34540(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7662647
• NCBI GeneID: 9791
• NCBI Accession #: NP_055569.1
• NCBI GenBank Nucleotide #: NM_014754.2
• UniProt Accession #: P48651
• Molecular Weight: 55,528 Da
• NCBI Official Full Name: phosphatidylserine synthase 1 isoform 1
• NCBI Official Synonym Full Names: phosphatidylserine synthase 1
• NCBI Official Symbol: PTDSS1
• NCBI Official Synonym Symbols: LMHD; PSS1; PSSA
• NCBI Protein Information: phosphatidylserine synthase 1; PSS-1; ptdSer synthase 1; serine-exchange enzyme I
• UniProt Protein Name: Phosphatidylserine synthase 1
• Protein Family: Phosphatidylserine synthase
• UniProt Gene Name: PTDSS1
• UniProt Synonym Gene Names: KIAA0024; PSSA; PSS-1; PtdSer synthase 1

NCBI Description

The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

PTDSS1: Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine. In membranes, PTDSS1 catalyzes mainly the conversion of phosphatidylcholine. Also converts, in vitro and to a lesser extent, phosphatidylethanolamine. Belongs to the phosphatidyl serine synthase family.

Protein type: EC 2.7.8.29; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: transferase activity

Biological Process: phosphatidylserine biosynthetic process

Disease: Lenz-majewski Hyperostotic Dwarfism