MLH1 sirna

MLH1 siRNA (Human)

Cat. Num. AAA8215328

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MLH1; MLH1 siRNA (Human); COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MLH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 756

• Applicable Applications for MLH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MLH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MLH1 sirna

siRNA to inhibit MLH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557757
• NCBI GeneID: 4292
• NCBI Accession #: NP_000240.1
• NCBI GenBank Nucleotide #: NM_000249.3
• UniProt Accession #: P40692; B4DI13; B4DQ11; E9PCU2
• Molecular Weight: 73,837 Da
• NCBI Official Full Name: DNA mismatch repair protein Mlh1 isoform 1
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2
• UniProt Entry Name: MLH1_HUMAN

NCBI Description

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.

Protein type: Tumor suppressor; DNA repair, damage

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: MutLalpha complex; male germ cell nucleus; nucleoplasm; chiasma; membrane; synaptonemal complex; nucleus

Molecular Function: protein binding; guanine/thymine mispair binding; ATPase activity; MutSalpha complex binding; single-stranded DNA binding; ATP binding

Biological Process: oogenesis; poly(A) tail shortening; meiotic metaphase I plate congression; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; somatic hypermutation of immunoglobulin genes; synapsis; isotype switching; male meiosis chromosome segregation; negative regulation of mitotic recombination; resolution of meiotic joint molecules as recombinants; spermatogenesis; spindle midzone assembly involved in meiosis; DNA repair; double-strand break repair via nonhomologous end joining

Disease: Muir-torre Syndrome; Mismatch Repair Cancer Syndrome; Colorectal Cancer, Hereditary Nonpolyposis, Type 2