MGAM sirna

MGAM siRNA (Human)

Cat. Num. AAA8227783

• Gene Names: MGAM; MG; MGA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MGAM; MGAM siRNA (Human); MGA; MGAML; Maltase-glucoamylase. intestinal; MGAM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MGAM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,857

• Applicable Applications for MGAM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MGAM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MGAM sirna

siRNA to inhibit MGAM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 221316699
• NCBI GeneID: 8972
• NCBI Accession #: NP_004659.2
• NCBI GenBank Nucleotide #: NM_004668.2
• UniProt Accession #: O43451; Q0VAX6; Q75ME7; Q86UM5
• Molecular Weight: 209,852 Da
• NCBI Official Full Name: maltase-glucoamylase, intestinal
• NCBI Official Synonym Full Names: maltase-glucoamylase
• NCBI Official Symbol: MGAM
• NCBI Official Synonym Symbols: MG; MGA
• NCBI Protein Information: maltase-glucoamylase, intestinal
• UniProt Protein Name: Maltase-glucoamylase, intestinal
• Protein Family: Maltase-glucoamylase
• UniProt Gene Name: MGAM
• UniProt Synonym Gene Names: MGA; MGAML
• UniProt Entry Name: MGA_HUMAN

NCBI Description

This gene encodes maltase-glucoamylase, which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase, but the proteins are only 59% homologous. Both are members of glycosyl hydrolase family 31, which has a variety of substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

MGAM: May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing. Belongs to the glycosyl hydrolase 31 family.

Protein type: EC 3.2.1.20; Carbohydrate Metabolism - galactose; Membrane protein, integral; EC 3.2.1.3; Carbohydrate Metabolism - starch and sucrose; Hydrolase

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: alpha-glucosidase activity; glucan 1,4-alpha-glucosidase activity; amylase activity; maltase activity; carbohydrate binding; catalytic activity

Biological Process: polysaccharide digestion; carbohydrate metabolic process; pathogenesis; starch catabolic process