MEMO1 sirna

MEMO1 siRNA (Human)

Cat. Num. AAA8210763

• Gene Names: MEMO1; MEMO; C2orf4; CGI-27; NS5ATP7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MEMO1; MEMO1 siRNA (Human); C2orf4; MEMO; NS5ATP7; Protein MEMO1; C21orf19-like protein; Hepatitis C virus NS5A-transactivated protein 7; HCV NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Mediator of cell motility 1; Memo-1; MEMO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MEMO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 274

• Applicable Applications for MEMO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MEMO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MEMO1 sirna

siRNA to inhibit MEMO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 212286159
• NCBI GeneID: 51072
• NCBI Accession #: NP_001131074.1
• NCBI GenBank Nucleotide #: NM_001137602.2
• UniProt Accession #: Q9Y316; Q5R2V8; Q5R2V9; Q6NSL5; B4DLS0; D6W575
• Molecular Weight: 34,294 Da
• NCBI Official Full Name: protein MEMO1 isoform 2
• NCBI Official Synonym Full Names: mediator of cell motility 1
• NCBI Official Symbol: MEMO1
• NCBI Official Synonym Symbols: MEMO; C2orf4; CGI-27; NS5ATP7
• NCBI Protein Information: protein MEMO1
• UniProt Protein Name: Protein MEMO1
• Protein Family: MEMO1 family protein
• UniProt Gene Name: MEMO1
• UniProt Synonym Gene Names: C2orf4; MEMO; NS5ATP7; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; Memo-1
• UniProt Entry Name: MEMO1_HUMAN

Uniprot Description

MEMO1: a highly conserved protein that controls cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Regulates directionality during HER2-dependent cell migration. Controls microtubules, the actin network, and adhesion site formation in migrating cells via the MEMO1-RHOA-DIAPH1 signaling pathway. Homologous to class III nonheme iron-dependent dioxygenases. No metal-binding or enzymatic activity has been detected. Binds HER2 phosphorylated at Y1222 via its vestigial enzymatic active site. Binds to and influences insulin-like growth factor-I (IRS1) receptor-dependent signaling events in breast carcinogenesis. Influences the epithelial-mesenchymal transition (EMT) via the IRS1/PI3K/Akt/Snail1 pathway. Interacts with cofilin, and influences PLCg1 and cofilin activities. Increased expression is associated with cancer aggressiveness. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Two isoform of the human protein are produced by alternatuve splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: cytosol; nucleus

Molecular Function: protein binding

Biological Process: regulation of microtubule-based process