Rabbit anti-Human PSMD9 Polyclonal Antibody | anti-PSMD9 antibody

PSMD9 Antibody

Cat. Num. AAA9402405

• Gene Names: PSMD9; p27; Rpn4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PSMD9; Polyclonal Antibody; PSMD9 Antibody; p27; Rpn4; anti-PSMD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PSMD9 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 118

• Applicable Applications for anti-PSMD9 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PSMD9.
• Target Name: PSMD9
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMD9 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using PSMD9 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PSMD9 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-PSMD9 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 386869374
• NCBI GeneID: 5715
• NCBI Accession #: NP_001248329.1
• NCBI GenBank Nucleotide #: NM_001261400.2
• UniProt Accession #: O00233; Q9BQ42; B2RD35; G3V1Q6
• Molecular Weight: 13,053 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 9 isoform 2
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 9
• NCBI Official Symbol: PSMD9
• NCBI Official Synonym Symbols: p27; Rpn4
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 9
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 9
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD9
• UniProt Entry Name: PSMD9_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

PSMD9: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Belongs to the proteasome subunit p27 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Motility/polarity/chemotaxis; Proteasome complex

Chromosomal Location of Human Ortholog: 12q24.31-q24.32

Cellular Component: nucleoplasm; proteasome regulatory particle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; bHLH transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of insulin secretion; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis