MCEE sirna

MCEE siRNA (Human)

Cat. Num. AAA8203200

• Gene Names: MCEE; GLOD2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MCEE; MCEE siRNA (Human); Methylmalonyl-CoA epimerase mitochondrial; DL-methylmalonyl-CoA racemase; MCEE sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MCEE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 176

• Applicable Applications for MCEE sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MCEE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MCEE sirna

siRNA to inhibit MCEE expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188035928
• NCBI GeneID: 84693
• NCBI Accession #: NP_115990.3
• NCBI GenBank Nucleotide #: NM_032601.3
• UniProt Accession #: Q96PE7; Q53TP1; Q8WW63
• Molecular Weight: 18,749 Da
• NCBI Official Full Name: methylmalonyl-CoA epimerase, mitochondrial
• NCBI Official Synonym Full Names: methylmalonyl CoA epimerase
• NCBI Official Symbol: MCEE
• NCBI Official Synonym Symbols: GLOD2
• NCBI Protein Information: methylmalonyl-CoA epimerase, mitochondrial
• UniProt Protein Name: Methylmalonyl-CoA epimerase, mitochondrial
• UniProt Gene Name: MCEE
• UniProt Entry Name: MCEE_HUMAN

NCBI Description

The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. [provided by RefSeq, Jul 2008]

Uniprot Description

MCEE: Defects in MCEE are a cause of methylmalonyl-CoA epimerase deficiency (MCEED); also known as methylmalonyl-CoA racemase deficiency or methylmalonic aciduria type 3. MCEE deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. Belongs to the glyoxalase I family.

Protein type: Isomerase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 5.1.99.1; Mitochondrial; Carbohydrate Metabolism - propanoate

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: mitochondrial matrix

Molecular Function: metal ion binding; methylmalonyl-CoA epimerase activity

Biological Process: L-methylmalonyl-CoA metabolic process; fatty acid beta-oxidation; short-chain fatty acid catabolic process; cellular lipid metabolic process

Disease: Methylmalonyl-coa Epimerase Deficiency