MAPKAPK5 sirna

MAPKAPK5 siRNA (Human)

Cat. Num. AAA8205138

• Gene Names: MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPKAPK5; MAPKAPK5 siRNA (Human); PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; MAPKAPK5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAPKAPK5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 471

• Applicable Applications for MAPKAPK5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAPKAPK5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPKAPK5 sirna

siRNA to inhibit MAPKAPK5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21237765
• NCBI GeneID: 8550
• NCBI Accession #: NP_003659.2
• NCBI GenBank Nucleotide #: NM_003668.3
• UniProt Accession #: Q8IW41; O60491; Q86X46; Q9BVX9; Q9UG86; B3KVA5
• Molecular Weight: 54,035 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 5 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 5
• NCBI Official Symbol: MAPKAPK5
• NCBI Official Synonym Symbols: MK5; MK-5; PRAK; MAPKAP-K5
• NCBI Protein Information: MAP kinase-activated protein kinase 5
• UniProt Protein Name: MAP kinase-activated protein kinase 5
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK5
• UniProt Synonym Gene Names: PRAK; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; PRAK
• UniProt Entry Name: MAPK5_HUMAN

NCBI Description

The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]

Uniprot Description

MAPKAPK5: a member of the MAPKAPK family of protein kinases. Activated through phosphorylation by MAP kinases including ERK, p38-alpha, and MAPp38-beta In response to cellular stress and proinflammatory cytokines. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Protein type: Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; p53 binding; mitogen-activated protein kinase binding; ATP binding

Biological Process: regulation of translation; negative regulation of TOR signaling pathway; activation of MAPK activity; protein amino acid autophosphorylation; Ras protein signal transduction; MAPKKK cascade; signal transduction