MAK sirna

MAK siRNA (Human)

Cat. Num. AAA8203948

• Gene Names: MAK; RP62; dJ417M14.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAK; MAK siRNA (Human); Serine/threonine-protein kinase MAK; Male germ cell-associated kinase; MAK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 583

• Applicable Applications for MAK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAK sirna

siRNA to inhibit MAK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 334724436
• NCBI GeneID: 4117
• NCBI Accession #: NP_001229314.1
• NCBI GenBank Nucleotide #: NM_001242385.1
• UniProt Accession #: P20794; Q547D0; Q9NUH7; F1T0K6; G1FL29
• Molecular Weight: 66,345 Da
• NCBI Official Full Name: serine/threonine-protein kinase MAK isoform 2
• NCBI Official Synonym Full Names: male germ cell-associated kinase
• NCBI Official Symbol: MAK
• NCBI Official Synonym Symbols: RP62; dJ417M14.2
• NCBI Protein Information: serine/threonine-protein kinase MAK
• UniProt Protein Name: Serine/threonine-protein kinase MAK
• Protein Family: Makatoxin
• UniProt Gene Name: MAK
• UniProt Entry Name: MAK_HUMAN

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.

Protein type: EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Nuclear receptor co-regulator; CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: centrosome; photoreceptor outer segment; photoreceptor inner segment; cytoplasm; midbody; nucleus

Molecular Function: protein binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of cell cycle; multicellular organismal development; protein amino acid autophosphorylation; photoreceptor cell maintenance; spermatogenesis; cell differentiation; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 62