MACF1 sirna

MACF1 siRNA (Human)

Cat. Num. AAA829045

• Gene Names: MACF1; ACF7; MACF; OFC4; ABP620
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MACF1; MACF1 siRNA (Human); ABP620; ACF7; KIAA0465; KIAA1251; Microtubule-actin cross-linking factor 1 isoforms 1/2/3/5; 620 kDa actin-binding protein; Actin cross-linking family protein 7; Macrophin-1; Trabeculin-alpha; MACF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MACF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 5430

• Applicable Applications for MACF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MACF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MACF1 sirna

siRNA to inhibit MACF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33188445
• NCBI GeneID: 23499
• NCBI Accession #: NP_036222.3
• NCBI GenBank Nucleotide #: NM_012090.5
• UniProt Accession #: Q9UPN3; O75053; Q5VW20; Q8WXY1; Q8WXY2; Q96PK2; Q9H540; Q9UKP0; Q9ULG9; B1ALC5; E9PJT0
• Molecular Weight: 670,137 Da
• NCBI Official Full Name: microtubule-actin cross-linking factor 1
• NCBI Official Synonym Full Names: microtubule-actin crosslinking factor 1
• NCBI Official Symbol: MACF1
• NCBI Official Synonym Symbols: ACF7; MACF; OFC4; ABP620
• NCBI Protein Information: microtubule-actin cross-linking factor 1
• UniProt Protein Name: Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5
• Protein Family: Microtubule-actin cross-linking factor
• UniProt Gene Name: MACF1
• UniProt Synonym Gene Names: ABP620; ACF7; KIAA0465; KIAA1251; ABP620
• UniProt Entry Name: MACF1_HUMAN

NCBI Description

This gene encodes a large protein containing numerous spectrin and leucine-rich repeat (LRR) domains. The encoded protein is a member of a family of proteins that form bridges between different cytoskeletal elements. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. Alternative splicing results in multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]

Uniprot Description

ACF7: a nuclear WD40-repeat protein. An F-actin-binding protein which may play a role in cross- linking actin to other cytoskeletal proteins. Also binds to microtubules. WD40 proteins are known to function as adaptors in pre-mrna processing and cytoskeleton assembly.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; microtubule; cytoskeleton; cytoplasm; plasma membrane; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; microtubule binding; ATPase activity; calcium ion binding; actin binding

Biological Process: regulation of microtubule-based process; Wnt receptor signaling pathway; mesoderm formation; wound healing; metabolic process; positive regulation of axon extension; Golgi to plasma membrane protein transport; regulation of focal adhesion formation; establishment and/or maintenance of cell polarity; posttranslational protein targeting to membrane; cell cycle arrest; positive regulation of Wnt receptor signaling pathway