LNX1 sirna

LNX1 siRNA (Human)

Cat. Num. AAA8221942

• Gene Names: LNX1; LNX; MPDZ; PDZRN2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LNX1; LNX1 siRNA (Human); LNX; PDZRN2; E3 ubiquitin-protein ligase LNX; Ligand of Numb-protein X 1; Numb-binding protein 1; PDZ domain-containing RING finger protein 2; LNX1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LNX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 728

• Applicable Applications for LNX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LNX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LNX1 sirna

siRNA to inhibit LNX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 187607085
• NCBI GeneID: 84708
• NCBI Accession #: NP_001119800.1
• NCBI GenBank Nucleotide #: NM_001126328.2
• UniProt Accession #: Q8TBB1; Q4W5K7; Q8N4C2; Q96MJ7; Q9BY20
• Molecular Weight: 69,643 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase LNX isoform a
• NCBI Official Synonym Full Names: ligand of numb-protein X 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: LNX1
• NCBI Official Synonym Symbols: LNX; MPDZ; PDZRN2
• NCBI Protein Information: E3 ubiquitin-protein ligase LNX
• UniProt Protein Name: E3 ubiquitin-protein ligase LNX
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: LNX1
• UniProt Synonym Gene Names: LNX; PDZRN2
• UniProt Entry Name: LNX1_HUMAN

NCBI Description

This gene encodes a membrane-bound protein that is involved in signal transduction and protein interactions. The encoded product is an E3 ubiquitin-protein ligase, which mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. This protein may play an important role in tumorogenesis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene, which is located on chromosome 17, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LNX1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Adaptor/scaffold; Ligase; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: cytoplasm

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; PDZ domain binding; ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein homooligomerization