LNPEP sirna

LNPEP siRNA (Human)

Cat. Num. AAA8234800

• Gene Names: LNPEP; CAP; IRAP; PLAP; P-LAP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LNPEP; LNPEP siRNA (Human); OTASE; Leucyl-cystinyl aminopeptidase; Cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP; Oxytocinase; OTase; Placental leucine aminopeptidase; P-LAP; LNPEP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LNPEP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1025

• Applicable Applications for LNPEP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LNPEP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LNPEP sirna

siRNA to inhibit LNPEP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61742777
• NCBI GeneID: 4012
• NCBI Accession #: NP_005566.2
• NCBI GenBank Nucleotide #: NM_005575.2
• UniProt Accession #: Q9UIQ6; O00769; Q15145; Q59H76; Q9TNQ2; Q9TNQ3; Q9UIQ7
• Molecular Weight: 115,062 Da
• NCBI Official Full Name: leucyl-cystinyl aminopeptidase isoform 1
• NCBI Official Synonym Full Names: leucyl/cystinyl aminopeptidase
• NCBI Official Symbol: LNPEP
• NCBI Official Synonym Symbols: CAP; IRAP; PLAP; P-LAP
• NCBI Protein Information: leucyl-cystinyl aminopeptidase
• UniProt Protein Name: Leucyl-cystinyl aminopeptidase
• Protein Family: Leucyl-cystinyl aminopeptidase
• UniProt Gene Name: LNPEP
• UniProt Synonym Gene Names: OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP
• UniProt Entry Name: LCAP_HUMAN

NCBI Description

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAP: a leucyl/cystinyl aminopeptidase. A type II membrane protein and secreted. Resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. Three alternatively spliced isoforms have been described.

Protein type: EC 3.4.11.3; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: cytoplasmic vesicle membrane; membrane; perinuclear region of cytoplasm; lysosomal membrane; integral to plasma membrane; plasma membrane; extracellular region; intracellular; cytosol

Molecular Function: zinc ion binding; metallopeptidase activity; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; cell-cell signaling; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein catabolic process; female pregnancy; proteolysis