LMBRD1 sirna

LMBRD1 siRNA (Human)

Cat. Num. AAA8200183

• Gene Names: LMBRD1; NESI; LMBD1; MAHCF; C6orf209
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LMBRD1; LMBRD1 siRNA (Human); C6orf209; NESI; Probable lysosomal cobalamin transporter; HDAg-L-interacting protein NESI; LMBR1 domain-containing protein 1; Nuclear export signal-interacting protein; LMBRD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LMBRD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 540

• Applicable Applications for LMBRD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LMBRD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LMBRD1 sirna

siRNA to inhibit LMBRD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 261878497
• NCBI GeneID: 55788
• NCBI Accession #: NP_060838.3
• NCBI GenBank Nucleotide #: NM_018368.3
• UniProt Accession #: Q9NUN5; Q5VUN6; Q86Y70; Q96FW4; Q9BY56; Q9NZD6; A8K204; E1P531
• Molecular Weight: 21,394 Da
• NCBI Official Full Name: probable lysosomal cobalamin transporter
• NCBI Official Synonym Full Names: LMBR1 domain containing 1
• NCBI Official Symbol: LMBRD1
• NCBI Official Synonym Symbols: NESI; LMBD1; MAHCF; C6orf209
• NCBI Protein Information: probable lysosomal cobalamin transporter
• UniProt Protein Name: Probable lysosomal cobalamin transporter
• UniProt Gene Name: LMBRD1
• UniProt Synonym Gene Names: C6orf209; NESI
• UniProt Entry Name: LMBD1_HUMAN

NCBI Description

This gene encodes a lysosomal membrane protein that may be involved in the transport and metabolism of cobalamin. This protein also interacts with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F.[provided by RefSeq, Oct 2009]

Uniprot Description

LMBRD1: Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors. Isoform 3 may play a role in the assembly of hepatitis delta virus (HDV). Defects in LMBRD1 are the cause of methylmalonic aciduria and homocystinuria type cblF (MMAHCF). A disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). It is due to accumulation of free cobalamin in lysosomes, thus hindering its conversion to cofactors. Clinical features include developmental delay, stomatitis, glossitis, seizures and methylmalonic aciduria responsive to vitamin B12. Belongs to the LIMR family. LMBRD1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; clathrin-coated endocytic vesicle

Molecular Function: cobalamin binding; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of protein kinase B signaling cascade; vitamin metabolic process; viral reproduction; cobalamin metabolic process; negative regulation of insulin receptor signaling pathway; water-soluble vitamin metabolic process

Disease: Methylmalonic Aciduria And Homocystinuria, Cblf Type