LIPA sirna

LIPA siRNA (Human)

Cat. Num. AAA8201355

• Gene Names: LIPA; LAL; CESD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LIPA; LIPA siRNA (Human); Lysosomal acid lipase/cholesteryl ester hydrolase; Acid cholesteryl ester hydrolase; LAL; Cholesteryl esterase; Lipase A; Sterol esterase; LIPA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LIPA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 399

• Applicable Applications for LIPA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIPA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LIPA sirna

siRNA to inhibit LIPA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51317399
• NCBI GeneID: 3988
• NCBI Accession #: NP_000226.2
• NCBI GenBank Nucleotide #: NM_000235.3
• UniProt Accession #: P38571; Q16529; Q53H21; Q5T074; Q5T771; Q96EJ0; B2RBH5; D3DR29
• Molecular Weight: 39,112 Da
• NCBI Official Full Name: lysosomal acid lipase/cholesteryl ester hydrolase isoform 1
• NCBI Official Synonym Full Names: lipase A, lysosomal acid, cholesterol esterase
• NCBI Official Symbol: LIPA
• NCBI Official Synonym Symbols: LAL; CESD
• NCBI Protein Information: lysosomal acid lipase/cholesteryl ester hydrolase
• UniProt Protein Name: Lysosomal acid lipase/cholesteryl ester hydrolase
• Protein Family: Lipase
• UniProt Gene Name: LIPA
• UniProt Synonym Gene Names: Acid cholesteryl ester hydrolase; LAL
• UniProt Entry Name: LICH_HUMAN

NCBI Description

This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

LIPA: Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor- mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. Defects in LIPA are the cause of Wolman disease (WOD). WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year. Defects in LIPA are the cause of cholesteryl ester storage disease (CESD). CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - steroid biosynthesis; Hydrolase; EC 3.1.1.13

Chromosomal Location of Human Ortholog: 10q23.2-q23.3

Cellular Component: lysosome

Molecular Function: sterol esterase activity; lipase activity

Biological Process: cell proliferation; homeostasis of number of cells within a tissue; tissue remodeling; cell morphogenesis; cytokine production; inflammatory response; lipid catabolic process; lung development

Disease: Lysosomal Acid Lipase Deficiency