Mouse Semaphorin 4D (SEMA4D) ELISA Kit | SEMA4D elisa kit

Mouse Semaphorin 4D (SEMA4D) ELISA Kit

Cat. Num. AAA2703297

• Gene Names: SEMA4D; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Mouse
• Synonyms: Semaphorin 4D (SEMA4D); Mouse Semaphorin 4D (SEMA4D) ELISA Kit; CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D; SEMA4D elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D).; No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed.
• Sequence Length: 862

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA4D elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 214010218
• NCBI GeneID: 10507
• NCBI Accession #: NP_006369.3
• NCBI GenBank Nucleotide #: NM_006378.3
• UniProt Accession #: Q92854; Q7Z5S4; Q8N8B0; B2RPM6
• Molecular Weight: 82,164 Da
• NCBI Official Full Name: semaphorin-4D isoform 1
• NCBI Official Synonym Full Names: semaphorin 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral component of plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; regulation of cell projection organization; regulation of cell shape; regulation of dendrite morphogenesis