LIN54 sirna

LIN54 siRNA (Human)

Cat. Num. AAA8225117

• Gene Names: LIN54; JC8.6; CXCDC1; MIP120
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LIN54; LIN54 siRNA (Human); CXCDC1; KIAA2037; Protein lin-54 homolog; CXC domain-containing protein 1; LIN54 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LIN54 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 528

• Applicable Applications for LIN54 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIN54 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LIN54 sirna

siRNA to inhibit LIN54 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 169234721
• NCBI GeneID: 132660
• NCBI Accession #: NP_001108479.1
• NCBI GenBank Nucleotide #: NM_001115007.2
• UniProt Accession #: Q6MZP7; Q32M68; Q32M69; Q6N071; Q76B60
• Molecular Weight: 25,743 Da
• NCBI Official Full Name: protein lin-54 homolog isoform b
• NCBI Official Synonym Full Names: lin-54 DREAM MuvB core complex component
• NCBI Official Symbol: LIN54
• NCBI Official Synonym Symbols: JC8.6; CXCDC1; MIP120
• NCBI Protein Information: protein lin-54 homolog
• UniProt Protein Name: Protein lin-54 homolog
• Protein Family: Protein
• UniProt Gene Name: LIN54
• UniProt Synonym Gene Names: CXCDC1; KIAA2037
• UniProt Entry Name: LIN54_HUMAN

NCBI Description

LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes (Schmit et al., 2009 [PubMed 19725879]).[supplied by OMIM, Dec 2010]

Uniprot Description

DKFZp686L1814: Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner. Belongs to the lin-54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 4q21.22

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of cell cycle; mitotic cell cycle; G2/M transition of mitotic cell cycle