LIMA1 sirna

LIMA1 siRNA (Human)

Cat. Num. AAA8218417

• Gene Names: LIMA1; EPLIN; SREBP3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LIMA1; LIMA1 siRNA (Human); EPLIN; SREBP3; LIM domain and actin-binding protein 1; Epithelial protein lost in neoplasm; LIMA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LIMA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 760

• Applicable Applications for LIMA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIMA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LIMA1 sirna

siRNA to inhibit LIMA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 165905589
• NCBI GeneID: 51474
• NCBI Accession #: NP_001107018.1
• NCBI GenBank Nucleotide #: NM_001113546.1
• UniProt Accession #: Q9UHB6; Q2TAN7; Q59FE8; Q9BVF2; Q9H8J1; Q9HBN5; Q9NX96; Q9NXC3; Q9NXU6; Q9P0H8; Q9UHB5; B2RB09
• Molecular Weight: 67,117 Da
• NCBI Official Full Name: LIM domain and actin-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain and actin binding 1
• NCBI Official Symbol: LIMA1
• NCBI Official Synonym Symbols: EPLIN; SREBP3
• NCBI Protein Information: LIM domain and actin-binding protein 1
• UniProt Protein Name: LIM domain and actin-binding protein 1
• Protein Family: LIM domain and actin-binding protein
• UniProt Gene Name: LIMA1
• UniProt Synonym Gene Names: EPLIN; SREBP3
• UniProt Entry Name: LIMA1_HUMAN

NCBI Description

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Uniprot Description

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; stress fiber; plasma membrane; actin cytoskeleton; cleavage furrow

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization