LARS2 sirna

LARS2 siRNA (Human)

Cat. Num. AAA8221366

• Gene Names: LARS2; LEURS; PRLTS4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LARS2; LARS2 siRNA (Human); KIAA0028; Probable leucine--tRNA ligase mitochondrial; Leucyl-tRNA synthetase; LeuRS; LARS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LARS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 903

• Applicable Applications for LARS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LARS2 sirna

siRNA to inhibit LARS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7661872
• NCBI GeneID: 23395
• NCBI Accession #: NP_056155.1
• NCBI GenBank Nucleotide #: NM_015340.3
• UniProt Accession #: Q15031
• Molecular Weight: 101,976 Da
• NCBI Official Full Name: probable leucine--tRNA ligase, mitochondrial
• NCBI Official Synonym Full Names: leucyl-tRNA synthetase 2, mitochondrial
• NCBI Official Symbol: LARS2
• NCBI Official Synonym Symbols: LEURS; PRLTS4
• NCBI Protein Information: probable leucine--tRNA ligase, mitochondrial
• UniProt Protein Name: Probable leucine--tRNA ligase, mitochondrial
• Protein Family: Probable leucine--tRNA ligase
• UniProt Gene Name: LARS2
• UniProt Synonym Gene Names: KIAA0028; LeuRS
• UniProt Entry Name: SYLM_HUMAN

NCBI Description

This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq, Jul 2008]

Uniprot Description

LARS2: a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq, Jul 2008]

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 6.1.1.4; Mitochondrial; Translation; Ligase

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytosol

Molecular Function: leucine-tRNA ligase activity; ATP binding

Biological Process: leucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; regulation of translational fidelity; gene expression

Disease: Perrault Syndrome 4