LAMTOR1 sirna

LAMTOR1 siRNA (Human)

Cat. Num. AAA8222056

• Gene Names: LAMTOR1; p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LAMTOR1; LAMTOR1 siRNA (Human); C11orf59; PDRO; Ragulator complex protein LAMTOR1; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; Lipid raft adaptor protein p18; Protein associated with DRMs and endosomes; p27Kip1-releasing factor from RhoA; p27RF-Rho; LAMTOR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LAMTOR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for LAMTOR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LAMTOR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LAMTOR1 sirna

siRNA to inhibit LAMTOR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8923579
• NCBI GeneID: 55004
• NCBI Accession #: NP_060377.1
• NCBI GenBank Nucleotide #: NM_017907.2
• UniProt Accession #: Q6IAA8; Q8WZ09; Q9NWT0
• Molecular Weight: 17,745 Da
• NCBI Official Full Name: ragulator complex protein LAMTOR1
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
• NCBI Official Symbol: LAMTOR1
• NCBI Official Synonym Symbols: p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• NCBI Protein Information: ragulator complex protein LAMTOR1
• UniProt Protein Name: Ragulator complex protein LAMTOR1
• Protein Family: Ragulator complex protein
• UniProt Gene Name: LAMTOR1
• UniProt Synonym Gene Names: C11orf59; PDRO; p27RF-Rho
• UniProt Entry Name: LTOR1_HUMAN

Uniprot Description

LAMTOR1: Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. Part of the Ragulator complex composed of LAMTOR1, LAMTOR2 and LAMTOR3. Interacts with LAMTOR2 and LAMTOR3; the interaction is direct. The Ragulator complex interacts with both the mTORC1 complex and heterodimers of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD. Interacts with RRAGB and RRAGD; the interaction is direct indicating that it probably constitutes the main RAG-interacting subunit of the Ragulator complex. Interacts with MMP14. Interacts with CDKN1B; prevents the interaction of CDKN1B with RHOA leaving RHOA in a form accessible to activation by ARHGEF2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; lysosome; lysosomal membrane; late endosome membrane; plasma membrane; lipid raft

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; protein complex scaffold

Biological Process: cholesterol homeostasis; endosome organization and biogenesis; lysosome localization; positive regulation of MAPKKK cascade; lysosome organization and biogenesis; regulation of receptor recycling; cell growth; endosome localization; positive regulation of TOR signaling pathway; positive regulation of GTPase activity