Rabbit Atg12 Polyclonal Antibody | anti-Atg12 antibody

Atg12

Cat. Num. AAA191007

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Atg12; Polyclonal Antibody; anti-Atg12 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human Atg12.
• Form/Format: Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 74

• Applicable Applications for anti-Atg12 antibody: Immunoblotting (IB), Immunofluorescence (IF)
• Application Notes: Immunoblotting: use at 1-2ug/ml. Bands of ~55kDa, corresponding to Atg12-Atg5 complex, and ~15kda, corresponding to free Atg12, are detected.; Immunofluorescence: use at 10ug/ml.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Synthetic peptide corresponding to amino acids at the C-terminus of human Atg12.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Immunofluorescence (IF)

Related Product Information for anti-Atg12 antibody

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of Atg13 and ULK1, and the regulation of the Atg13-ULK1-RB1CC1 complex. Under starvation conditions, Atg13 is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylation of Atg13 depends on nutrient conditions; it is dephosphorylated during starvation or following treatment with rapamycin. Three isoforms of the human protein are produced by alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 480540330
• NCBI GeneID: 9140
• NCBI Accession #: NP_001264712.1
• NCBI GenBank Nucleotide #: NM_001277783.1
• UniProt Accession #: O94817; Q6PJV2
• Molecular Weight: 7,746 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12 isoform 2
• NCBI Official Synonym Full Names: autophagy related 12
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L; APG12-like

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q22.3

Cellular Component: cytosol; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: APG8 conjugating enzyme activity; protein binding

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; macroautophagy; mitochondrion degradation