KMT2E sirna

KMT2E siRNA (Human)

Cat. Num. AAA8240590

• Gene Names: KMT2E; MLL5; NKp44L; HDCMC04P
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KMT2E; KMT2E siRNA (Human); MLL5; Histone-lysine N-methyltransferase 2E; Lysine N-methyltransferase 2E; Myeloid/lymphoid or mixed-lineage leukemia protein 5; KMT2E sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KMT2E siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1858

• Applicable Applications for KMT2E sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KMT2E gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KMT2E sirna

siRNA to inhibit KMT2E expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 91199543
• NCBI GeneID: 55904
• NCBI Accession #: NP_061152.3
• NCBI GenBank Nucleotide #: NM_018682.3
• UniProt Accession #: Q8IZD2; Q6P5Y2; Q6PKG4; Q6T316; Q86TI3; Q86W12; Q86WG0; Q86WL2; Q8IV78; B6ZDE4; B6ZDM3; M4K8J3
• Molecular Weight: 131,737 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase 2E
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2E
• NCBI Official Symbol: KMT2E
• NCBI Official Synonym Symbols: MLL5; NKp44L; HDCMC04P
• NCBI Protein Information: histone-lysine N-methyltransferase 2E
• UniProt Protein Name: Histone-lysine N-methyltransferase 2E
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: KMT2E
• UniProt Synonym Gene Names: MLL5; Lysine N-methyltransferase 2E
• UniProt Entry Name: KMT2E_HUMAN

NCBI Description

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.1.1.43; Transcription, coactivator/corepressor; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nuclear speck

Molecular Function: protein binding; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity

Biological Process: retinoic acid receptor signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; neutrophil activation; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; histone H3-K4 methylation; neutrophil mediated immunity; DNA methylation; erythrocyte differentiation; cell cycle arrest