KIF14 sirna

KIF14 siRNA (Human)

Cat. Num. AAA8225086

• Gene Names: KIF14; MKS12
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KIF14; KIF14 siRNA (Human); KIAA0042; Kinesin-like protein KIF14; KIF14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KIF14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1157

• Applicable Applications for KIF14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KIF14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KIF14 sirna

siRNA to inhibit KIF14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 795881084
• NCBI GeneID: 9928
• NCBI Accession #: NP_001292721.1
• NCBI GenBank Nucleotide #: NM_001305792.1
• UniProt Accession #: Q15058; Q14CI8; Q4G0A5; Q5T1W3
• Molecular Weight: 186,492 Da
• NCBI Official Full Name: kinesin-like protein KIF14 isoform 2
• NCBI Official Synonym Full Names: kinesin family member 14
• NCBI Official Symbol: KIF14
• NCBI Official Synonym Symbols: MKS12
• NCBI Protein Information: kinesin-like protein KIF14
• UniProt Protein Name: Kinesin-like protein KIF14
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF14
• UniProt Entry Name: KIF14_HUMAN

NCBI Description

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: microtubule; kinesin complex; membrane; cytoplasm; plasma membrane; spindle midzone; midbody; nucleus

Molecular Function: tubulin binding; protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding; PDZ domain binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of myelination; cell proliferation in forebrain; regulation of cell migration; microtubule-based movement; cerebellar cortex development; establishment of protein localization; regulation of G2/M transition of mitotic cell cycle; regulation of neuron apoptosis; positive regulation of cell proliferation; regulation of cell growth; negative regulation of neuron apoptosis; regulation of Rap protein signal transduction; regulation of cell adhesion; positive regulation of cytokinesis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; cerebellar granular layer structural organization; olfactory bulb development; hippocampus development; negative regulation of integrin activation; cytoskeleton-dependent intracellular transport; mitotic metaphase plate congression; activation of protein kinase activity; microtubule depolymerization; cerebral cortex development; cerebellar Purkinje cell layer structural organization; negative regulation of apoptosis

Disease: Meckel Syndrome 12