KCNN3 sirna

KCNN3 siRNA (Human)

Cat. Num. AAA8225279

• Gene Names: KCNN3; SK3; hSK3; SKCA3; KCa2.3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNN3; KCNN3 siRNA (Human); K3; Small conductance calcium-activated potassium channel protein 3; SK3; SKCa 3; SKCa3; KCa2.3; KCNN3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNN3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 746

• Applicable Applications for KCNN3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNN3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNN3 sirna

siRNA to inhibit KCNN3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 323276683
• NCBI GeneID: 3782
• NCBI Accession #: NP_001191016.1
• NCBI GenBank Nucleotide #: NM_001204087.1
• UniProt Accession #: Q9UGI6; O43517; Q86VF9; Q8WXG7; B1ANX0
• Molecular Weight: 47,093 Da
• NCBI Official Full Name: small conductance calcium-activated potassium channel protein 3 isoform c
• NCBI Official Synonym Full Names: potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 3
• NCBI Official Symbol: KCNN3
• NCBI Official Synonym Symbols: SK3; hSK3; SKCA3; KCa2.3
• NCBI Protein Information: small conductance calcium-activated potassium channel protein 3
• UniProt Protein Name: Small conductance calcium-activated potassium channel protein 3
• Protein Family: Small conductance calcium-activated potassium channel protein
• UniProt Gene Name: KCNN3
• UniProt Synonym Gene Names: K3; SK3; SKCa 3; SKCa3
• UniProt Entry Name: KCNN3_HUMAN

NCBI Description

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

KCNN3: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell soma; integral to membrane; plasma membrane

Molecular Function: calmodulin binding; small conductance calcium-activated potassium channel activity

Biological Process: synaptic transmission