KCNK10 sirna

KCNK10 siRNA (Human)

Cat. Num. AAA8211684

• Gene Names: KCNK10; TREK2; TREK-2; K2p10.1; PPP1R97
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNK10; KCNK10 siRNA (Human); TREK2; Potassium channel subfamily K member 10; Outward rectifying potassium channel protein TREK-2; TREK-2 K(+) channel subunit; KCNK10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNK10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 538

• Applicable Applications for KCNK10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNK10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNK10 sirna

siRNA to inhibit KCNK10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 10863961
• NCBI GeneID: 54207
• NCBI Accession #: NP_066984.1
• NCBI GenBank Nucleotide #: NM_021161.4
• UniProt Accession #: P57789; Q6B014; Q8TDK7; Q8TDK8; Q9HB59; B2R8T4; B2RCT3; B5TJL4
• Molecular Weight: 60,335 Da
• NCBI Official Full Name: potassium channel subfamily K member 10 isoform 1
• NCBI Official Synonym Full Names: potassium channel, two pore domain subfamily K, member 10
• NCBI Official Symbol: KCNK10
• NCBI Official Synonym Symbols: TREK2; TREK-2; K2p10.1; PPP1R97
• NCBI Protein Information: potassium channel subfamily K member 10
• UniProt Protein Name: Potassium channel subfamily K member 10
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK10
• UniProt Synonym Gene Names: TREK2
• UniProt Entry Name: KCNKA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TREK-2: Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, potassium

Chromosomal Location of Human Ortholog: 14q31.3

Cellular Component: plasma membrane; integral to membrane

Molecular Function: potassium channel activity; voltage-gated ion channel activity

Biological Process: synaptic transmission; transport; signal transduction