KAT2A sirna

KAT2A siRNA (Human)

Cat. Num. AAA8214359

• Gene Names: KAT2A; GCN5; hGCN5; GCN5L2; PCAF-b
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KAT2A; KAT2A siRNA (Human); GCN5; GCN5L2; HGCN5; Histone acetyltransferase KAT2A; General control of amino acid synthesis protein 5-like 2; Histone acetyltransferase GCN5; HsGCN5; Lysine acetyltransferase 2A; STAF97; KAT2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KAT2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 837

• Applicable Applications for KAT2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KAT2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KAT2A sirna

siRNA to inhibit KAT2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153791535
• NCBI GeneID: 2648
• NCBI Accession #: NP_066564.2
• NCBI GenBank Nucleotide #: NM_021078.2
• UniProt Accession #: Q92830; Q8N1A2; Q9UCW1
• Molecular Weight: 48,921 Da
• NCBI Official Full Name: histone acetyltransferase KAT2A
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 2A
• NCBI Official Symbol: KAT2A
• NCBI Official Synonym Symbols: GCN5; hGCN5; GCN5L2; PCAF-b
• NCBI Protein Information: histone acetyltransferase KAT2A
• UniProt Protein Name: Histone acetyltransferase KAT2A
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: KAT2A
• UniProt Synonym Gene Names: GCN5; GCN5L2; HGCN5; HsGCN5
• UniProt Entry Name: KAT2A_HUMAN

NCBI Description

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]

Uniprot Description

GCN5: an ubiquitous histone acetyltransferase that promotes transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Interacts with P300, CBP, ADA2, and is a component of the TFTC-HAT complex. Two splice variant isoforms have been described.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; extracellular space

Molecular Function: protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; chromatin binding; H3 histone acetyltransferase activity

Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; cell proliferation; establishment and/or maintenance of chromatin architecture; somitogenesis; telencephalon development; viral reproduction; in utero embryonic development; multicellular organism growth; neural tube closure; midbrain development; regulation of protein stability; metencephalon development; histone deubiquitination