JAM3 sirna

JAM3 siRNA (Human)

Cat. Num. AAA8223532

• Gene Names: JAM3; JAMC; JAM-2; JAM-3; JAM-C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: JAM3; JAM3 siRNA (Human); Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; JAM3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: JAM3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 259

• Applicable Applications for JAM3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human JAM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for JAM3 sirna

siRNA to inhibit JAM3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 328927057
• NCBI GeneID: 83700
• NCBI Accession #: NP_001192258.1
• NCBI GenBank Nucleotide #: NM_001205329.1
• UniProt Accession #: Q9BX67; Q8WWL8; Q96FL1; B3KWG9
• Molecular Weight: 29,223 Da
• NCBI Official Full Name: junctional adhesion molecule C isoform 2
• NCBI Official Synonym Full Names: junctional adhesion molecule 3
• NCBI Official Symbol: JAM3
• NCBI Official Synonym Symbols: JAMC; JAM-2; JAM-3; JAM-C
• NCBI Protein Information: junctional adhesion molecule C
• UniProt Protein Name: Junctional adhesion molecule C
• Protein Family: Junctional adhesion molecule
• UniProt Gene Name: JAM3
• UniProt Synonym Gene Names: JAM-C; JAM-3
• UniProt Entry Name: JAM3_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

JAM3: Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC). A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: Golgi apparatus; desmosome; extracellular space; tight junction; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: myelination; adaptive immune response; extracellular matrix organization and biogenesis; myeloid progenitor cell differentiation; establishment of cell polarity; axon regeneration; cell-matrix adhesion; transmission of nerve impulse; angiogenesis; blood coagulation; neutrophil homeostasis; leukocyte migration; spermatid development; leukocyte migration during inflammatory response

Disease: Hemorrhagic Destruction Of The Brain, Subependymal Calcification, And Cataracts