ITGA7 sirna

ITGA7 siRNA (Human)

Cat. Num. AAA8216787

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGA7; ITGA7 siRNA (Human); Integrin alpha-7; ITGA7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ITGA7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,044

• Applicable Applications for ITGA7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGA7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGA7 sirna

siRNA to inhibit ITGA7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 222418613
• NCBI GeneID: 3679
• NCBI Accession #: NP_001138468.1
• NCBI GenBank Nucleotide #: NM_001144996.1
• UniProt Accession #: Q13683; O43197; Q86W93; Q9NY89; Q9UET0; Q9UEV2; B4E3U0; C9JMD3; C9JMZ6
• Molecular Weight: 114,545 Da
• NCBI Official Full Name: integrin alpha-7 isoform 1
• NCBI Official Synonym Full Names: integrin, alpha 7
• NCBI Official Symbol: ITGA7
• NCBI Protein Information: integrin alpha-7
• UniProt Protein Name: Integrin alpha-7
• Protein Family: Integrin
• UniProt Gene Name: ITGA7
• UniProt Entry Name: ITA7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. They mediate a wide spectrum of cell-cell and cell-matrix interactions, and thus play a role in cell migration, morphologic development, differentiation, and metastasis. This protein functions as a receptor for the basement membrane protein laminin-1. It is mainly expressed in skeletal and cardiac muscles and may be involved in differentiation and migration processes during myogenesis. Defects in this gene are associated with congenital myopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

ITGA7: Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation. Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI). A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures. Belongs to the integrin alpha chain family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: muscle tendon junction; cell surface; cytoplasm; plasma membrane; neuromuscular junction; sarcolemma

Molecular Function: integrin binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; regulation of cell shape; cell migration; muscle development; skeletal muscle development; extracellular matrix organization and biogenesis; cell-matrix adhesion; blood vessel morphogenesis

Disease: Muscular Dystrophy, Congenital, Due To Integrin Alpha-7 Deficiency