ITGA2 sirna

ITGA2 siRNA (Human)

Cat. Num. AAA829377

• Gene Names: ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGA2; ITGA2 siRNA (Human); CD49B; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD antigen CD49b; ITGA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ITGA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1181

• Applicable Applications for ITGA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGA2 sirna

siRNA to inhibit ITGA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116295258
• NCBI GeneID: 3673
• NCBI Accession #: NP_002194.2
• NCBI GenBank Nucleotide #: NM_002203.3
• UniProt Accession #: P17301; Q14595
• Molecular Weight: 129,295 Da
• NCBI Official Full Name: integrin alpha-2
• NCBI Official Synonym Full Names: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• NCBI Official Symbol: ITGA2
• NCBI Official Synonym Symbols: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• NCBI Protein Information: integrin alpha-2
• UniProt Protein Name: Integrin alpha-2
• Protein Family: Integrin
• UniProt Gene Name: ITGA2
• UniProt Synonym Gene Names: CD49B; GPIa
• UniProt Entry Name: ITA2_HUMAN

NCBI Description

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cell surface; focal adhesion; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane

Molecular Function: viral receptor activity; collagen binding; integrin binding; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; entry of virus into host cell; response to muscle activity; extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; positive regulation of smooth muscle cell proliferation; positive regulation of leukocyte migration; cell-matrix adhesion; positive regulation of collagen binding; positive regulation of collagen biosynthetic process; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to organic cyclic substance; response to L-ascorbic acid; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; response to drug; substrate-bound cell migration; integrin-mediated signaling pathway; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; cell proliferation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; positive regulation of phagocytosis, engulfment; blood coagulation; positive regulation of inflammatory response; positive regulation of transmission of nerve impulse; response to amine stimulus

Disease: Bleeding Disorder, Platelet-type, 9