ITGA1 sirna

ITGA1 siRNA (Human)

Cat. Num. AAA8238498

• Gene Names: ITGA1; VLA1; CD49a
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGA1; ITGA1 siRNA (Human); Integrin alpha-1; CD49 antigen-like family member A; Laminin and collagen receptor; VLA-1; CD antigen CD49a; ITGA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ITGA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1179

• Applicable Applications for ITGA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGA1 sirna

siRNA to inhibit ITGA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31657142
• NCBI GeneID: 3672
• NCBI Accession #: NP_852478.1
• NCBI GenBank Nucleotide #: NM_181501.1
• UniProt Accession #: P56199; B2RNU0
• Molecular Weight: 130,848 Da
• NCBI Official Full Name: integrin alpha-1
• NCBI Official Synonym Full Names: integrin, alpha 1
• NCBI Official Symbol: ITGA1
• NCBI Official Synonym Symbols: VLA1; CD49a
• NCBI Protein Information: integrin alpha-1
• UniProt Protein Name: Integrin alpha-1
• Protein Family: Integrin
• UniProt Gene Name: ITGA1
• UniProt Entry Name: ITA1_HUMAN

NCBI Description

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA1: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: neuron projection; cell surface; focal adhesion; membrane; acrosome; plasma membrane; perikaryon; integrin complex; external side of plasma membrane; lipid raft

Molecular Function: collagen binding; protein binding; metal ion binding; protein phosphatase binding; receptor binding

Biological Process: integrin-mediated signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of phosphoprotein phosphatase activity; neutrophil chemotaxis; axon guidance; extracellular matrix organization and biogenesis; activation of MAPK activity; cell-matrix adhesion; vasodilation; negative regulation of cell proliferation; cellular extravasation; muscle contraction; positive regulation of neuron apoptosis; blood coagulation