Human Integrin Alpha 1 (ITGa1) ELISA Kit | ITGa1 elisa kit

Human Integrin Alpha 1 (ITGa1) ELISA Kit

Cat. Num. AAA2022992

• Gene Names: ITGA1; VLA1; CD49a
• Reactivity: Human
• Synonyms: Integrin Alpha 1 (ITGa1); Human Integrin Alpha 1 (ITGa1) ELISA Kit; CD49-A; CD49a; ITG-A1; VLA1; CD49 antigen-like family member A; Laminin and collagen receptor; ITGa1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 1 (ITGa1).; No significant cross-reactivity or interference between Integrin Alpha 1 (ITGa1) and analogues was observed.
• Sequence Length: 1,179

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 29pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 1 (ITGa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 1 (ITGa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 1 (ITGa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 1 (ITGa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ITGa1 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 31657142
• NCBI GeneID: 3672
• NCBI Accession #: NP_852478.1
• NCBI GenBank Nucleotide #: NM_181501.1
• UniProt Accession #: P56199; B2RNU0
• Molecular Weight: 130,848 Da
• NCBI Official Full Name: integrin alpha-1
• NCBI Official Synonym Full Names: integrin, alpha 1
• NCBI Official Symbol: ITGA1
• NCBI Official Synonym Symbols: VLA1; CD49a
• NCBI Protein Information: integrin alpha-1
• UniProt Protein Name: Integrin alpha-1
• Protein Family: Integrin
• UniProt Gene Name: ITGA1
• UniProt Entry Name: ITA1_HUMAN

NCBI Description

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA1: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: neuron projection; cell surface; focal adhesion; membrane; acrosome; plasma membrane; perikaryon; integrin complex; external side of plasma membrane; lipid raft

Molecular Function: collagen binding; protein binding; metal ion binding; protein phosphatase binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; neutrophil chemotaxis; axon guidance; extracellular matrix organization and biogenesis; activation of MAPK activity; cell-matrix adhesion; vasodilation; negative regulation of cell proliferation; muscle contraction; cellular extravasation; positive regulation of neuron apoptosis; blood coagulation