IREB2 sirna

IREB2 siRNA (Human)

Cat. Num. AAA8230707

• Gene Names: IREB2; ACO3; IRP2; IRP2AD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IREB2; IREB2 siRNA (Human); Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2; IREB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IREB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 963

• Applicable Applications for IREB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IREB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IREB2 sirna

siRNA to inhibit IREB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41352693
• NCBI GeneID: 3658
• NCBI Accession #: NP_004127.1
• NCBI GenBank Nucleotide #: NM_004136.2
• UniProt Accession #: P48200; Q13095; Q1HE21; Q59FQ7; Q8WVK6; Q9UF17; A8KAC7; E1CJT9; H0YKU0
• Molecular Weight: 37,487 Da
• NCBI Official Full Name: iron-responsive element-binding protein 2
• NCBI Official Synonym Full Names: iron-responsive element binding protein 2
• NCBI Official Symbol: IREB2
• NCBI Official Synonym Symbols: ACO3; IRP2; IRP2AD
• NCBI Protein Information: iron-responsive element-binding protein 2
• UniProt Protein Name: Iron-responsive element-binding protein 2
• Protein Family: Iron-responsive element-binding protein
• UniProt Gene Name: IREB2
• UniProt Synonym Gene Names: IRE-BP 2; IRP2
• UniProt Entry Name: IREB2_HUMAN

Uniprot Description

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding

Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; response to iron(II) ion; osteoclast differentiation; protoporphyrinogen IX biosynthetic process; iron ion transport; intestinal absorption; aging; post-embryonic development